Page 3 example the apparent molecular weights of the protein bands in benchmark prestained protein ladder are shown below. Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue, orange, and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and. Find and order markers and products like this briteruler prestained protein ladder 3 colors on order product abin629432. Thermo scientific pageruler plus prestained protein. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and. M protein molecular mass marker pageruler unstained protein. Hxstable prestained protein ladder dna protein ladders. Pageruler plus prestained protein ladder, 10 to 250 kda. Product information thermo scientific pageruler plus.
Fermentas sm0671 pdf labaid labaid, pageblue, pageruler, pagesilver are fermentas trademarks. The thermo scientific pageruler plus prestained protein ladders are a mixture of nine recombinant proteins ranging from 10 kda to 250 kda. The protein ladder is conveniently packaged and ready to use with no heating, diluting or additional. Prestained protein ladders and unstained protein ladders abcam. Your search returned 77 protein ladders prestained ladders and. Monitor protein migrationsample in sdspage, monitor protein transfer onto membranes during western blotting, sizing of proteins on sdspage and western blots introduction prestained protein ladder v is a threecolor protein standard with 10 prestained proteins covering a wide range of molecular weights for 10 to 180 kda. Thermo scientific pageruler prestained protein ladder. Proteins are covalently coupled with a blue chromophore except for two reference bands one green and one red band at 25 kda and 75 kda respectively made visible when separated on. Thermo scientific pierce protein molecular weight marker. Cst prestained protein marker, broad range 11190 kda. Each lot of prestained protein standards is individually calibrated for estimating the mw of sample proteins. The accuruler prestained protein ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kda. The protein ladder is supplied in a readytouse format for direct loading on. Prestained protein marker, broad range 11190 kda is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kda following electrophoresis.
For protein staining coomassie brilliant blue was used. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine blue, orange, and greenstained proteins 10 to 250 kda for use as size standards in protein electrophoresis sdspage and western blotting. The thermo scientific pageruler unstained protein ladder is a mixture of 14 recombinant, highly purified, unstained proteins 10 kda to 200 kda for use as size standards in protein electrophoresis sdspage and western blotting. Numerous studies have investigated the activity of maitake polysaccharide extracts, but studies of maitake proteins are scarce. Load the following volumes of the ladder on sdspage gel. Prestained protein ladder, broad range 10230 kda neb. Characterization of a novel maitake grifola frondosa.
It is designed for monitoring protein separated during. Does pierce pageruler prestained protein ladder a mix of. Thermo scientific pageruler unstained broad range protein. Opal prestained protein standard 10180kda mb2090500. The thermo scientific pageruler unstained protein ladder is a mixture of 14 recombinant, highly purified, unstained proteins. The thermo scientific pageruler unstained protein ladder consists of a unstained mixture of 14 recombinant, highly purified proteins ranging from 10 kda to 200 kda. Biotinylated protein ladder detection pack is designed to detect the molecular weight ladders on western blots when using the horseradish peroxidase hrp based western detection system. Page 2 directions the benchmark prestained protein ladder is supplied in a readytouse format. Effect of tomato variety, cultivation, climate and processing. Prestained natural protein standards for sdspage and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to assess transfer efficiency on blots. Thermo scientific pageruler plus prestained protein ladder, 10 to 250 kda mixture of 9 blue, orange and greenstained proteins 10 to 250kda used as size standards in protein electrophoresis.
Thermo scientific pageruler prestained protein ladder, 10. Mabs produced in mammalian cells are secreted into the cell culture supernatant, where formation of aggregates can be. Numerous studies have investigated the activity of maitake polysaccharide extracts, but. Unstained versus prestained protein ladders scientist.
Protein marker prestained protein ladder ps11 geneon. Effect of tomato variety, cultivation, climate and. Pageruler prestained protein ladder thermo scientific was used as molecular weight marker. Pageruler prestained protein ladder 10 170 kda migration patterns were determined using respective nupage precast gels. For accurate protein sizing on sdspolyacrylamide gels and western blots. Unstained protein ladders are more accurate for sizing proteins, as. Prestained protein ladders and unstained protein ladders. Mix thoroughly to ensure the solution is homogeneous. Pageruler prestained protein ladder pageruler plus prestained protein ladder prestained protein molecular weight marker unstained protein 816% trisglycine sdspage gel blot gel blot protein. Secbased analysis of mab aggregates in cell culture supernatant. Read 5 answers by scientists with 2 recommendations from their colleagues to the question asked by hu chengcheng on mar 29, 2016. With, one of our best sellers, 3 tricolored prestained protein marker, you can load up over 300 wells for western blotting application.
Five microliters of benchmark prestained protein ladder was separated on a. Page 4 benchmark prestained protein ladder limited use. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis. The thermo scientific pageruler prestained protein ladder is a prestained mixture of ten recombinant proteins ranging from 10 kda to 180 kda. Prestained protein ladder, broad range 10230 kda is a mixture of highly purified recombinant proteins covalently coupled to a blue dye that resolves into 12 sharp bands when electrophoresed. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis sdspage and western blotting. Thermo scientific pageruler prestained protein ladder, 10 to 180 kda mixture of 10 blue, orange and greenstained proteins 10 to 180kda used as size standards in protein electrophoresis sdspage and western blotting. Product information thermo scientific pageruler unstained protein. Product information thermo scientific pageruler unstained. The protein ladder is supplied in a readytouse format for direct loading onto ge.
Thaw the ladder either at room temperature or at 3740c for a few minutes to dissolve precipitated solids. Genscript offers a wide selection of protein molecular weight standards for sdspage and western blot, including prestained, unstained, peptide, westernviewable, and other standards. The readytouse bluestain protein ladder covers a molecular weight between 24510 kda, and is used for protein electrophoresis operations. Benchmark prestained protein ladder must show 10 major bands when separated by electrophoresis on a novex 420% trisglycine gel. Protein ladders and standards markers life science. Unstained versus prestained protein ladders scientist solutions. Protein molecular weight marker ps11 prestained is ideal for precise sizing of proteins by sdspage and for western blotting. In this study, we purified and characterized a new g. The bluelf prestained protein ladder is a threecolor protein standard with prestained proteins covering a wide range molecular weights from 3.
The protein ladder is supplied in a readytouse format for direct loading. Opal prestained protein standard 10180kda is a threecolor protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180kda. In addition, the ladder proteins except for the 5kda peptide contain an integral streptag ii sequence and may be detected on western blots using streptactin conjugates. Bluestain protein ladder, 11245 kda goldbio asset 1. Pageruler prestained protein ladder pageruler plus prestained protein ladder prestained protein molecular weight marker unstained protein molecular weight marker 816% trisglycine sdspage. Page 4 benchmark prestained protein ladder limited use label.
Gfp is a nonglucan heterodimeric 83 kda protein that. The pageruler unstained broad range protein ladder is supplied readytouse. The protein concentrations are carefully balanced for even intensity. Briteruler prestained protein ladder 3colors abin629432. Color prestained protein standard, broad range 10250 kda. The protein mw markers in this ladder resolve into sharp bands.
Thermo scientific pageruler prestained protein ladder, 10 to 180 kda mixture of 10 blue, orange and greenstained proteins 10 to 180kda used as size standards in protein electrophoresis sds. Thermo scientific pageruler plus prestained protein ladder. Compare prestained protein ladders from leading suppliers on biocompare. Thermo scientific pageruler plus prestained protein ladder is a mixture of nine 9 blue, orange and greenstained proteins 10 to 250kda for use as size standards in protein electrophoresis sdspage. The protein ladder is conveniently packaged and ready to. Prestained protein marker, broad range 11190 kda is a mixture of purified proteins covalently coupled to a blue dye that resolve to a series of 11 bands between 11 and 190 kda. The bluelf prestained protein ladder is designed for monitoring protein separation during sdspage, verification of western transfer efficiency on membranes pvdf, nylon, or nitrocellulose and for approximating the size of p roteins. Specificationsfeatures pageruler prestained protein ladder is a mixture of ten blue, orange, and greenstained recombinant proteins 10 to 170 kda for use as size standards in protein electrophoresis sds. The prestained protein standards consist of colored bands for easy identification and allow you to monitor the progress of an electrophoretic run. Prestained natural protein standards for sdspage and western blotting provide a quick and easy way to monitor protein separation during electrophoresis and to.
Prestained protein marker, broad range 7175 kda neb. Prestained protein ladder v is a threecolor protein standard with 10 prestained proteins covering a wide range of molecular weights for 10 to 180 kda. Comparison of multicolor prestained precision plus protein. The apparent molecular weight of the prestained proteins are given in the table below. Specificationsfeatures pageruler prestained protein ladder is a mixture of ten blue, orange, and greenstained recombinant proteins 10 to 170 kda for use as size standards in protein electrophoresis sdspage and western blotting. Product information thermo scientific pageruler prestained. Introducing thermo scientific pierce prestained and. I found that pierce pageruler prestained protein ladder26616 was also detected. However, an unstained protein ladder can only be visualized following staining with coomassie, or a similar nonspecific protein stain. The ladder is supplied in gel loading buffer and is ready to use. Thermo scientific pageruler prestained protein ladder is a mixture of 10 blue, orange, and greenstained proteins 10 to 180 kda for use as size standards in protein electrophoresis sdspage and western blotting. Comparison of multicolor prestained protein standards precision plus protein prestained all blue, dual color, and kaleidoscope standards offer matching migration patterns and nonshifting bands for every color. The apparent molecular weight of each protein kda has been determined by calibration against an unstained protein ladder in each electrophoresis conditions.
The apparent molecular weight of each protein kda has. The hxstable prestained protein ladder is a threecolor protein standard with 12 prestained proteins covering a wide range molecular weights from 10 to 245 kda. Grifola frondosa, also known as maitake, is a culinary mushroom with immuneenhancing and antitumor effects. Prestained protein ladders can have a sligtly different mobility and mass than the same peotein ladder which is not prestained. Direct analysis of mab aggregates in mammalian cell. Excelband 3color prestained protein ladder, 10 kda 245 kda, pm5100, 500ulpack. M protein molecular mass marker pageruler unstained. Does pierce pageruler prestained protein ladder a mix of phosphorylated protein. The blueray prestained protein ladder is a threecolor protein standard with 10 prestained proteins covering a wide range molecular weights for 10 to 180 kilodalton kda. Pageruler prestained protein ladder 2 x 250 l from thermo. Three different chromophores are bound to the proteins, producing a brightly colored ladder.
Thermo scientific pageruler prestained nir protein ladder the pageruler prestained nir protein ladder is a mixture of 10 proteins 11 to 250kda that are bluestained and fluorlabeled for nearir fluorescent visualization and protein sizing. M protein molecular mass marker pageruler unstained protein ladder fermentas from biology 402 at peking university. The ladder is visualized by sdspage using coomassie or silver stains or detected in western blots with protein stains. Thermo scientific pageruler unstained protein ladder is a mixture of 14 recombinant, highly purified, unstained proteins 10 to 200 kda for use as size standards in protein electrophoresis sdspage and western blotting. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel.
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